| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Van Vreede, Olivia L (14)
|
NIVICT |
34.92 |
34.47 |
-0.45 |
| 2 |
Konz, Ashley L (14)
|
NIJETS |
38.89 |
34.75 |
-4.14 |
| 3 |
Kadlecik, Grace K (15)
|
NIISWM |
38.08 |
34.85 |
-3.23 |
| 4 |
Hrosar, Saya J (17)
|
NISYCH |
52.00 |
37.04 |
-14.96 |
| 5 |
von Holtz, Reatha E (14)
|
NIMETS |
39.32 |
37.90 |
-1.42 |
| 6 |
Heidrich, Xandria A (15)
|
NIUNAC |
36.26 |
38.05 |
1.79 |
| 7 |
Spencer, Hope V (16)
|
NICSCI |
|
39.98 |
- |
| 8 |
Cywinski, Anna C (10)
|
NIVICT |
41.21 |
40.53 |
-0.68 |
| 9 |
Lin, Isabella E (12)
|
NIVELO |
39.41 |
41.72 |
2.31 |
| 10 |
Gibson, Allison E (16)
|
NIKRKN |
|
41.82 |
- |
| 11 |
Janeski, Gabrielle M (11)
|
NIKRKN |
|
44.34 |
- |
| 12 |
Upcraft, Malia L (11)
|
NIMETS |
56.71 |
46.42 |
-10.29 |
| 13 |
Monteiro, Martha G (12)
|
NIVELO |
48.02 |
46.46 |
-1.56 |
| 14 |
Burnham, Madeline (9)
|
NIVELO |
47.80 |
46.96 |
-0.84 |
| 15 |
Paddock, Isabel I (14)
|
NIKRKN |
|
47.98 |
- |
| 16 |
Owczarzak, Madeline L (10)
|
NICSCI |
52.64 |
48.66 |
-3.98 |
| 16 |
Chen, Chloe Y (10)
|
NISDSC |
47.69 |
48.66 |
0.97 |
| 18 |
Onze, Emily S (11)
|
NIVICT |
49.50 |
48.76 |
-0.74 |
| 19 |
Kuksenkova, Maria M (10)
|
NIKRKN |
|
49.28 |
- |
| 20 |
Latessa, Rose G (12)
|
NICSCI |
54.81 |
50.23 |
-4.58 |
| 21 |
Ryan, Isabella A (9)
|
NIVELO |
48.60 |
50.42 |
1.82 |
| 22 |
Lapierre, Lydia F (13)
|
NIKRKN |
|
50.61 |
- |
| 23 |
Mayo, Mari E (11)
|
NIKRKN |
|
50.96 |
- |
| 24 |
Francescotti, Maria G (11)
|
NISYCH |
54.26 |
52.19 |
-2.07 |
| 25 |
Codjovi, Jelani A (9)
|
NIVELO |
49.42 |
53.02 |
3.60 |
| 26 |
Duell, Emma F (14)
|
NIISWM |
50.00 |
53.21 |
3.21 |
| 27 |
Chen, Sophie Y (8)
|
NISDSC |
49.27 |
53.53 |
4.26 |
| 28 |
Shortslef, Allison C (11)
|
NICSCI |
|
54.96 |
- |
| 29 |
Waters, Ava R (12)
|
NIKRKN |
|
57.26 |
- |
| 30 |
Wales, Thearyn J (9)
|
NISYCH |
1:02.46 |
1:02.20 |
-0.26 |
| 31 |
Mayo, Heather L (9)
|
NIKRKN |
1:07.86 |
1:07.89 |
0.03 |
|
DeLapp, Sydney R (14)
|
NIOLSC |
34.00 |
DQ |
- |